Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 9000-0756. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Prepare your i3/i3x instrument for shipment. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Figure 2. 1) EarlyTox Glutathione Assay (6. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the. 72 MB. We would like to show you a description here but the site won’t allow us. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The SpectraMax® M5/M5e Multimode Plate Reader is a high-performance microplate reader that can read volumes as low as 2uL and perform various detection modes for bioresearch and drug discovery applications. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Principle of the HTRF IP-One competitive binding assay. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Compatible with SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3 readers 0200-6191: Gray replacement validation plate case Compatible with. 3 or greater. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Find regional support. SpectraMax® Microplate Readers and SoftMax® Pro Software. After running the LM1 Plate protocol, not all values in the values in Results sections are displayed; what should I do? This is a SoftMax Pro software problem rather than anything specific to the LM1 plate, protocol or reader. The chamber. The CellTiter-Glo assay from Promega uses luciferase. 1. Digitizer Systems. 635. #6590-0137) • SpectraMax i3x Multi-Mode Microplate Reader • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. x and earlier. Our absorbance plate readers feature our PathCheck Sensor technology and. In . Sale. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Read More. Table of Contents. Add to Cart. OPTICSon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. 1. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. If you do not have an iLab account you must first make one: this application note, we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert assays. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. 2. Wide range of applications; Five preferred detection modes;The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 1) SpectraMax Glo Steady Luc Reporter Assay (6. i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3 Validation Plate 0200- Cuvette Absorbance SpectraMax Plus 384, ABS Plus, M2, M2e,. Sunnyvale, California -- October 5, 2017. We therefore gathered salient. The M5 has two PMTs. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. LoadingIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput. View the Molecular Devices websiteThis version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. View and Download Molecular Devices SpectraMax Plus 384 user manual online. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax® iD5. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. Materials. Transcreener ® ADP. Multimode Readers Comparison Chart. Printed in USA. , Sigma cat. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Enzyme dilution curves were performed for Lck, a tyrosine kinase withThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. FlexStation™ II. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. OPTICSThe trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 4. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Wavelength precision. In most cases we will be able to perform the repair as listed, however in rare instances, you may be required to ship the instrument into our Service Depot Facility. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Pick the best speed, sensitivity, and price for your needs. F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. On-site Repair Service available nationwide for Molecular Devices SpectraMax M5 Plate Reader's. 00 USD. Monday to Friday 8am - 5pm GMT. Fluorescence Microplate Reader Comparison Chart. Lower limits of detection for ATP determined using the ATPliteThe Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. When the user applies a set temperature of 37°C, the top is heated to 37. 1. DRAWER button Opens and closes the microplate drawer when the instrument is in idle state. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax® Aquamax. Agilent VWorks Automation Control soft-Only Molecular Devices microplate plate readers offer the capability to . SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. SpectraMax M5, Analyst HT or Analyst GT. Plot of the lower end of the DNA standard curve, ranging from 4. In May 2011, Molecular Devices’ SpectraMax M5e became the first microplate reader in space when NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. See SpectraMax® Microplate Reader User Manual Download Page. 306 plate air cal fail,low light. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Was the failure only of the Detection Estimate (Mag3) Test for the. M2. Table of Contents. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C) AccuBlue HiRange kit. Molecular Devices User Guide. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Turn on Plate Reader and wait. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. IMAP technology is based on. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. In the M2, M5 and Plus 384, the light. Order Now. The. These robust readers have been placed in labs from Antarctica to. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. 3. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. All other trademarks or registered trademarks are the property of their respective owners. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : Multimode Analysis. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. SpectraMax M Series (Molecular Devices) FlexStation 3 (Molecular Devices) Fluorescence Microplate Readers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. (“Molecular Devices”) equipment, software, reagents, and consumables to use in theWe would like to show you a description here but the site won’t allow us. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Table 2. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and. UV/Vis Photometric Performance: Wavelength Range. Near bottom. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 314 low lamp level warning. ABS Plus can run both. The SpectraMax M5 Multi-Mode Microplate Reader is manufactured by Molecular Devices. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Cavicide (Warehouse, Cat # 79300360) 6. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X We would like to show you a description here but the site won’t allow us. Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. -39. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!US HEADQUARTERS. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET assays. Published Dec 16, 2020 | Updated Dec 06, 2021. B Contact Us Phone: Japan (Osaka)+1-800-635-5577 Web: IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. More Product Information. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. 312 gain calibration failed. measure the depth (optical pathlength) of samples with no temperature. Phone: +1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. This manual is subject to change without notice. 3 mm. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Turn the reader on and secure the USB cable. Measuring cell health on the SpectraMax iD3 reader with cell viability assays. Examples of results are presented below for the LMax 384II , a dedicated, top-of-the-line microplate luminometer, the Analyst GT, a multimode filter-based instrument and the SpectraMax M5, a multi-detection, monochromator-based reader with both top- and bottom-read capability. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 6. To support these assays, we provide application notes as well as. HEALTH AND SAFETY CONSIDERAIONS SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 00 USD. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 7°C (gradient heating design). View the Molecular Devices website. , October 25, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of SpectraMax® M5, a five-mode. MaterialsThe calculated LOQ values were 0. It has a cuvette port, spectral scanning, and up to six wavelengths per read. industry-leading data acquisition and analysis tool SoftMax Pro, the SpectraMax i3x Detection Platform is the total solution for all your research needs. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The CyQUANT Cell Proliferation Assay is a quick, sensitive fluorescence method for quantitating cell numbers or cellular DNA content. STAKMAX. ©2022 Molecular Devices, LLC. . The built-in micro-volume sample port allows for working with sample volumes as small as 0. -49. Plate stacker and robot integration. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Computer Cables and connectors The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 0 out. The single mode Luminescence Readers (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities. STAKMAX. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. #0200-7011) Instrument setupSoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Assay Performance with Various Instrument. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orMolecular Devices. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Monday to Friday 7am - 5pm Pacific Time. performs within its specifications. 72 MB. 3. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. 5. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 0. Molecular Devices Corporation. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. #R7110) 。FAM标记的磷酸化多肽底物 (Molecular Devices Cat. The SpectraMax 190 reader can be integrated with Molecular . A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 3. Spectranet customer portal. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. Homogeneous Time-Resolved Fluorescence (HTRF) . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. Molecular Devices Spectramax M5 Microplate Reader; Related products. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Supplier: Molecular Devices. 6: bioNova científica, s. item 6 Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair $8,000. 5577. Table 2. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. SpectraMax M-series Microplate readers. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. We would like to show you a description here but the site won’t allow us. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Molecular Devices Spectramax M 5 Manual -. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 2. 005) Photometric PrecisionScanLater Western Blot Detection Cartridge. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. Here, we describe the setup of a bacterial growth assay using SoftMax ® Pro 7. Terms and Conditions Privacy Policy Online Terms of Use. Conventional Electrophysiology. The following document is intended to demonstrate setup of this instrument and provide representativeMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Home; Knowledge BaseMolecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Molecular Devices Spectramax M5 Microplate Reader Condition: Used, tested in working condition. Cavicide (Warehouse, Cat # 79300360) 6. It has a cuvette port, spectral scanning, and up to six wavelengths per read. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Molecular Devices, Inc. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Fortunately, Molecular Devices offers a system with variable bandwidth that can be specific and sensitive at the same time and is suitable for assay optimization, so you do not have to choose. Learn how SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for Molecular Devices' microplate. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Unplug the power cable from the back of the instrument. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. This document is provided to customers who have purchased Molecular Devices, Inc. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 2. NORTH AMERICA. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Plate chamber design of the SpectraMax category 1 microplate readers. Additional information is also available in the User Manuals. Representative data from three different SpectraMax Quant dsDNA Assay Kits. Other technologies such as imaging. White plates reflect the light, enabling even low levels of Luminescence to be detected. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. 3. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities. HTRF-compatible SpectraMax Multi-Mode Readers. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 We would like to show you a description here but the site won’t allow us. View the Molecular Devices websiteMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Mary A. es •. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Background fluorescence has been subtracted. View the Molecular Devices websiteThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Table of Contents. View the Molecular Devices websiteすべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 6. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Please refer to the Obsolete Microplate Reader Models for more information. keeping with best practices, reader performance should. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Lift up the lid over the chamber to insert or remove a cuvette. It has. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 2018. Molecular Devices, LLC. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Specifications subject to change without notice. 315 can't find zero order. Prepare your i3/i3x instrument for shipment. While they play an important role in generating data from a wide range of. 2), the fluorescence intensity of the FAM conjugated to the miR-148b mimic was measured in each well at 495/520 nm (Excitation/Emission) using a Molecular Devices Spectramax M5 Microplate/Cuvette Reader. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. +1 800-635-5577. This page. The following document is. SoftMax Pro Software can collect data from one or more microplates orSpectraMax ABS Plus SpectraTest ABS2 v1. edu) to be added as a trained user in iLab. Choose cables that meet the requirement described in this appendix. Published Dec 16, 2020 | Updated Dec 16, 2020. For life sciences research, the system is well suited. Homogeneous Time-Resolved Fluorescence (HTRF) . View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seWe would like to show you a description here but the site won’t allow us. : 915 515 403 • Fax: 914 334 545 • e-mail: [email protected] 1 1 10 10 100 100 1000 1000 10000 0. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. 4. Detection modalities are shown in Table 1-1. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, SpectraMax Plus 384 reader Why is the instrument keypad inactive? When connected to SoftMax Pro software during a run, the SpectraMax Series plate reader keypad will be inactivated for. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Check software compatibility with Windows 10 and locate your software installer. This integrated system provides users with a multi. . View the Molecular Devices website. SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. Spectranet customer portal. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. View the Molecular Devices websiteLearn how to compare dose-response curves where there is no direct measurement of a product using Parallel line analysis - a new feature from SoftMax Pro 7 software. The smaller PMT is. 7 are achievable. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. Figure 5. Instrument settings used with the SpectraMax M5 Microplate Reader. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax Detection Cartridge Fluorescence Polarization SpectraMax® Detection Cartridges offers great sensitivity. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next- BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Delivers single mode reader performance in one multimode reader package. CHO M1WT3 antagonist IC50 curves. 0 (or higher) Data Acquisition and Analysis Software. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SpectraMax Spectrofluorometers. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. For more information please visit us at: and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Our. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. SpectraMax DuoLuc Reporter Assay (SMP 6.